Clathrin-dependent entry of severe acute respiratory syndrome coronavirus into target cells expressing ACE2 with the cytoplasmic tail deleted
Identifieur interne : 003B83 ( Main/Exploration ); précédent : 003B82; suivant : 003B84Clathrin-dependent entry of severe acute respiratory syndrome coronavirus into target cells expressing ACE2 with the cytoplasmic tail deleted
Auteurs : Yuuki Inoue [Japon] ; Nobuyuki Tanaka [Japon] ; Yoshinori Tanaka [Japon] ; Shingo Inoue [Japon] ; Kouichi Morita [Japon] ; MIN ZHUANG [Japon] ; Toshio Hattori [Japon] ; Kazuo Sugamura [Japon]Source :
- Journal of virology [ 0022-538X ] ; 2007.
Descripteurs français
- KwdFr :
- Animaux, Cellules COS, Chlorpromazine (pharmacologie), Clathrine (antagonistes et inhibiteurs), Clathrine (génétique), Clathrine (métabolisme), Cytoplasme (enzymologie), Délétion de séquence, Endocytose (), Endosomes (virologie), Humains, Lignée cellulaire tumorale, Peptidyl-Dipeptidase A (génétique), Peptidyl-Dipeptidase A (métabolisme), Petit ARN interférent (), Pénétration virale (), Virus du SRAS (), Virus du SRAS (physiologie).
- MESH :
- antagonistes et inhibiteurs : Clathrine.
- enzymologie : Cytoplasme.
- génétique : Clathrine, Peptidyl-Dipeptidase A.
- métabolisme : Clathrine, Peptidyl-Dipeptidase A.
- pharmacologie : Chlorpromazine.
- physiologie : Virus du SRAS.
- virologie : Endosomes.
- Pascal (Inist)
English descriptors
- KwdEn :
- Acute, Animals, COS Cells, Cell Line, Tumor, Chlorocebus aethiops, Chlorpromazine (pharmacology), Clathrin, Clathrin (antagonists & inhibitors), Clathrin (genetics), Clathrin (metabolism), Coronavirus, Cytoplasm (enzymology), Endocytosis (drug effects), Endosomes (virology), Humans, Peptidyl-Dipeptidase A (genetics), Peptidyl-Dipeptidase A (metabolism), RNA, Small Interfering (drug effects), SARS Virus (drug effects), SARS Virus (physiology), Sequence Deletion, Target cell, Virology, Virus Internalization (drug effects).
- MESH :
- chemical , antagonists & inhibitors : Clathrin.
- chemical , drug effects : RNA, Small Interfering.
- chemical , genetics : Clathrin, Peptidyl-Dipeptidase A.
- chemical , metabolism : Clathrin, Peptidyl-Dipeptidase A.
- chemical , pharmacology : Chlorpromazine.
- drug effects : Endocytosis, SARS Virus, Virus Internalization.
- enzymology : Cytoplasm.
- physiology : SARS Virus.
- virology : Endosomes.
- Animals, COS Cells, Cell Line, Tumor, Chlorocebus aethiops, Humans, Sequence Deletion.
Abstract
The penetration of various viruses into host cells is accomplished by hijacking the host endocytosis machinery. In the case of severe acute respiratory syndrome coronavirus (SARS-CoV) infection, viral entry is reported to require a low pH in intracytoplasmic vesicles; however, little is known about how SARS-CoV invades such compartments. Here we demonstrate that SARS-CoV mainly utilizes the clathrin-mediated endocytosis pathway for its entry to target cells by using infectious SARS-CoV, as well as a SARS-CoV pseudovirus packaged in the SARS-CoV envelope. The SARS-CoV entered caveolin-1-negative HepG2 cells, and the entry was significantly inhibited by treatment with chlorpromazine, an inhibitor for clathrin-dependent endocytosis, and by small interfering RNA-mediated gene silencing for the clathrin heavy chain. Furthermore, the SARS-CoV entered COS7 cells transfected with the mutant of ACE2 with the cytoplasmic tail deleted, SARS-CoV receptor, as well as the wild-type ACE2, and their entries were significantly inhibited by treatment with chlorpromazine. In addition, ACE2 translocated into EEA1-positive early endosomes immediately after the virus attachment to ACE2. These results suggest that when SARS-CoV binds ACE2 it is internalized and penetrates early endosomes in a clathrin-dependent manner and that the cytoplasmic tail of ACE2 is not required for the penetration of SARS-CoV.
Url:
Affiliations:
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Le document en format XML
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University Graduate School of Medicine</s1><s2>Sendai 980-8575</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sendai 980-8575</wicri:noRegion></affiliation></author><author><name sortKey="Tanaka, Nobuyuki" sort="Tanaka, Nobuyuki" uniqKey="Tanaka N" first="Nobuyuki" last="Tanaka">Nobuyuki Tanaka</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology and Immunology, Internal Medicine, Tohoku University Graduate School of Medicine</s1><s2>Sendai 980-8575</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sendai 980-8575</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Division of Immunology, Miyagi Cancer Center Research Institute</s1><s2>Natori 981-1293</s2><s3>JPN</s3><sZ>2 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Natori 981-1293</wicri:noRegion></affiliation></author><author><name sortKey="Tanaka, Yoshinori" sort="Tanaka, Yoshinori" uniqKey="Tanaka Y" first="Yoshinori" last="Tanaka">Yoshinori Tanaka</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology and Immunology, Internal Medicine, Tohoku University Graduate School of Medicine</s1><s2>Sendai 980-8575</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sendai 980-8575</wicri:noRegion></affiliation></author><author><name sortKey="Inoue, Shingo" sort="Inoue, Shingo" uniqKey="Inoue S" first="Shingo" last="Inoue">Shingo Inoue</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Virology, Institute of Tropical Medicine, Nagasaki University</s1><s2>Nagasaki 852-8523</s2><s3>JPN</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Nagasaki 852-8523</wicri:noRegion></affiliation></author><author><name sortKey="Morita, Kouichi" sort="Morita, Kouichi" uniqKey="Morita K" first="Kouichi" last="Morita">Kouichi Morita</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Virology, Institute of Tropical Medicine, Nagasaki University</s1><s2>Nagasaki 852-8523</s2><s3>JPN</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Nagasaki 852-8523</wicri:noRegion></affiliation></author><author><name sortKey="Min Zhuang" sort="Min Zhuang" uniqKey="Min Zhuang" last="Min Zhuang">MIN ZHUANG</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Infectious and Respiratory Diseases, Internal Medicine, Tohoku University Graduate School of Medicine</s1><s2>Sendai 980-8575</s2><s3>JPN</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sendai 980-8575</wicri:noRegion></affiliation></author><author><name sortKey="Hattori, Toshio" sort="Hattori, Toshio" uniqKey="Hattori T" first="Toshio" last="Hattori">Toshio Hattori</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Infectious and Respiratory Diseases, Internal Medicine, Tohoku University Graduate School of Medicine</s1><s2>Sendai 980-8575</s2><s3>JPN</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sendai 980-8575</wicri:noRegion></affiliation></author><author><name sortKey="Sugamura, Kazuo" sort="Sugamura, Kazuo" uniqKey="Sugamura K" first="Kazuo" last="Sugamura">Kazuo Sugamura</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology and Immunology, Internal Medicine, Tohoku University Graduate School of Medicine</s1><s2>Sendai 980-8575</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sendai 980-8575</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acute</term><term>Animals</term><term>COS Cells</term><term>Cell Line, Tumor</term><term>Chlorocebus aethiops</term><term>Chlorpromazine (pharmacology)</term><term>Clathrin</term><term>Clathrin (antagonists & inhibitors)</term><term>Clathrin (genetics)</term><term>Clathrin (metabolism)</term><term>Coronavirus</term><term>Cytoplasm (enzymology)</term><term>Endocytosis (drug effects)</term><term>Endosomes (virology)</term><term>Humans</term><term>Peptidyl-Dipeptidase A (genetics)</term><term>Peptidyl-Dipeptidase A (metabolism)</term><term>RNA, Small Interfering (drug effects)</term><term>SARS Virus (drug effects)</term><term>SARS Virus (physiology)</term><term>Sequence Deletion</term><term>Target cell</term><term>Virology</term><term>Virus Internalization (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules COS</term><term>Chlorpromazine (pharmacologie)</term><term>Clathrine (antagonistes et inhibiteurs)</term><term>Clathrine (génétique)</term><term>Clathrine (métabolisme)</term><term>Cytoplasme (enzymologie)</term><term>Délétion de séquence</term><term>Endocytose ()</term><term>Endosomes (virologie)</term><term>Humains</term><term>Lignée cellulaire tumorale</term><term>Peptidyl-Dipeptidase A (génétique)</term><term>Peptidyl-Dipeptidase A (métabolisme)</term><term>Petit ARN interférent ()</term><term>Pénétration virale ()</term><term>Virus du SRAS ()</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Clathrin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>RNA, Small Interfering</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Clathrin</term><term>Peptidyl-Dipeptidase A</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Clathrin</term><term>Peptidyl-Dipeptidase A</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Chlorpromazine</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Clathrine</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Endocytosis</term><term>SARS Virus</term><term>Virus Internalization</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Cytoplasme</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Cytoplasm</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Clathrine</term><term>Peptidyl-Dipeptidase A</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Clathrine</term><term>Peptidyl-Dipeptidase A</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Chlorpromazine</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Endosomes</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Endosomes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>COS Cells</term><term>Cell Line, Tumor</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Sequence Deletion</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term><term>Cellules COS</term><term>Coronavirus</term><term>Clathrine</term><term>Aigu</term><term>Cellule cible</term><term>Délétion de séquence</term><term>Endocytose</term><term>Humains</term><term>Lignée cellulaire tumorale</term><term>Petit ARN interférent</term><term>Pénétration virale</term><term>Virologie</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The penetration of various viruses into host cells is accomplished by hijacking the host endocytosis machinery. In the case of severe acute respiratory syndrome coronavirus (SARS-CoV) infection, viral entry is reported to require a low pH in intracytoplasmic vesicles; however, little is known about how SARS-CoV invades such compartments. Here we demonstrate that SARS-CoV mainly utilizes the clathrin-mediated endocytosis pathway for its entry to target cells by using infectious SARS-CoV, as well as a SARS-CoV pseudovirus packaged in the SARS-CoV envelope. The SARS-CoV entered caveolin-1-negative HepG2 cells, and the entry was significantly inhibited by treatment with chlorpromazine, an inhibitor for clathrin-dependent endocytosis, and by small interfering RNA-mediated gene silencing for the clathrin heavy chain. Furthermore, the SARS-CoV entered COS7 cells transfected with the mutant of ACE2 with the cytoplasmic tail deleted, SARS-CoV receptor, as well as the wild-type ACE2, and their entries were significantly inhibited by treatment with chlorpromazine. In addition, ACE2 translocated into EEA1-positive early endosomes immediately after the virus attachment to ACE2. These results suggest that when SARS-CoV binds ACE2 it is internalized and penetrates early endosomes in a clathrin-dependent manner and that the cytoplasmic tail of ACE2 is not required for the penetration of SARS-CoV.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Inoue, Yuuki" sort="Inoue, Yuuki" uniqKey="Inoue Y" first="Yuuki" last="Inoue">Yuuki Inoue</name></noRegion><name sortKey="Hattori, Toshio" sort="Hattori, Toshio" uniqKey="Hattori T" first="Toshio" last="Hattori">Toshio Hattori</name><name sortKey="Inoue, Shingo" sort="Inoue, Shingo" uniqKey="Inoue S" first="Shingo" last="Inoue">Shingo Inoue</name><name sortKey="Min Zhuang" sort="Min Zhuang" uniqKey="Min Zhuang" last="Min Zhuang">MIN ZHUANG</name><name sortKey="Morita, Kouichi" sort="Morita, Kouichi" uniqKey="Morita K" first="Kouichi" last="Morita">Kouichi Morita</name><name sortKey="Sugamura, Kazuo" sort="Sugamura, Kazuo" uniqKey="Sugamura K" first="Kazuo" last="Sugamura">Kazuo Sugamura</name><name sortKey="Tanaka, Nobuyuki" sort="Tanaka, Nobuyuki" uniqKey="Tanaka N" first="Nobuyuki" last="Tanaka">Nobuyuki Tanaka</name><name sortKey="Tanaka, Nobuyuki" sort="Tanaka, Nobuyuki" uniqKey="Tanaka N" first="Nobuyuki" last="Tanaka">Nobuyuki Tanaka</name><name sortKey="Tanaka, Yoshinori" sort="Tanaka, Yoshinori" uniqKey="Tanaka Y" first="Yoshinori" last="Tanaka">Yoshinori Tanaka</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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